Accueil > Productions Scientifiques > Projets de recherche > Projets ANR terminés > Prohet ANR Défi H12

Tasks and Objectives

The objectives of the project Défi H12 are organized in three major axes :

A. Discover, adapt, identify, create and manage the microbial consortia implied at the anode of electrolyzers (tasks 1 and 2).
B. Increase the knowledge on the mechanisms of electro-microbial catalysis and conceive optimal electrochemically active biofilms (tasks 2 and 3).
C. Develop the engineering of MEC based on the fundamental knowledge acquired and evaluate the potential of the technology (tasks 4 and 5).

The project is then organized in five scientific tasks :

- Task 1. Discover and identify novel microbial consortia, compare and select them.

  • Select a “type inoculum” for further tasks.
  • Identify novel sources of electrochemically-active bacteria, compare their performances and choose a maximum of two consortia for studying in task 2.
  • Evaluate the capacity of adaptation of the microbial consortia, to the operational conditions of MEC.

- Task 2. Engineering of microbial consortia.

  • Optimization of the abiotic parameters for the consortia selected in task 1.
  • Follow the dynamics of microbial communities in electrochemically-active biofilms for long periods, under controlled conditions and under the effect of perturbations.
  • Improve the microbial consortia by different isolation methods, ecological engineering or adaptation.

- Task 3. Electrode engineering

  • Optimization of the cathode for hydrogen production in neutral pH media.
  • Progress into knowledge of microbial anode mechanisms and establish the kinetic laws for MEC calculation, for the “type consortium and conditions”.
  • Optimize microbial anodes for their specific utilization in MEC.

- Task 4. Electrolyzer engineering

  • Develop a MEC that integrates knowledge acquired in previous tasks.
  • Identify the best operational conditions and prepare the integration of MEC in the biomass transformation chain.

- Task 5. Coupling MEC and anaerobic digestion.

  • Integrate MEC technology in a sequence of bio-hydrogen production by anaerobic digestion, following two configurations :
    • Coupling in series, with MEC using the products of fermentation in a different reaction system
    • Integration of digestion process inside the MEC.
  • Propose a first eco-evaluation of both configurations.